Figure 2. Fisetin dampens the proliferation, migration along with infiltration of Patu-8988 cells. (A) Colony formation assessment of Patu-8988 cells inoculated with 0,50 and 100 μM fisetin. (B) CCK-8 assay of the HPNE, PANC-1 and Patu-8988 cells inoculated with 160 μM, 140 μM, 120 μM, 100 μM, 80 μM, 60 μM, 40 μM, and 20 μM of Fisetin or an equivalent DMEM medium volume for 48 h. (C) Ki67 Immunofluorescence staining of Patu-8988 cells inoculated with 0,50 and 100 μM fisetin for 24h. Bar = 50μm. (D) Histogram representing the number of colonies in each group. (E) Histogram illustrating Ki67 positive rate of Patu-8988 cells in every group. (F) Wound healing assays of Patu-8988 cells inoculated with 0, 50 and 100μM fisetin for 24 h. Bar = 500μm. (G) Histogram illustrating cell mobility of Patu-8988 cells in every group. (H) Transwell assays of Patu-8988 cells inoculated with 0, 50 and 100μM fisetin for 24 h. (I) Histogram illustrating the invasion cell number in every group. All experiments were replicated thrice, and data are given as means±SD.*p<.05, **p<.01, ***p<.001, ****p<.0001, in contrast with the controls.