Research Paper Volume 13, Issue 22 pp 24542—24559

Increased proliferation and differentiation capacity of placenta-derived mesenchymal stem cells from women of median maternal age correlates with telomere shortening

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Figure 5. Osteogenic differentiation ability of PDMSCs from different maternal age group. (A) Representative image of Osteogenic differentiation of all 5 age groups. Upper panel showed that undifferentiated cells did not retain Alizarin staining. Lower panel showed that differentiated cells were positive to Alizarin staining. Real Time PCR performed to measure the gene expression of (B) Osteocalcin, (C) Collagen type 1 (COL1), (D) Runt-related transcription factor 2 (RUNX2), and (E) Osteopontin (OPN) of all 5 age groups. Group A: 18-21; Group B: 22-25; Group C: 26-30; Group D: 31-35; Group E: 36 and over. *, p<0.05; **, p<0.01; ***, p<0.0005; ****, p<0.0001. Scale bar 200 μm.