Research Paper Volume 13, Issue 23 pp 25089—25105

Figure 3. Puerarin inhibits the tumor growth and metastasis of PDAC in the animal xenograft model. (A) Effects of puerarin on morphologic changes in the experimental groups. (B) Pathological results of H&E staining for PDAC in tissues of the model group. Bar = 50 μm. (C) Effect of puerarin on the volume of tumors in the animal xenograft model. (D) Effects of puerarin on tumor weight. (E) IHC staining for Ki67 in the puerarin-treated model. Bar = 50 μm. (F) IHC staining for c-Myc in the puerarin-treated model. Bar = 50 μm. (G) Protein expression of Cleaved caspase-8, Bax and Bcl-2 in PDACs in different groups. (H) IHC staining for Cleaved caspase-8 and cytochrome C in the puerarin-treated model. Bar = 50 μm. (I) IHC staining for E-cadherin and α-SMA in the puerarin-treated model. Bar = 50 μm. (J) IHC staining for HIF-1α in the puerarin-treated model. Bar = 50 μm. The data are presented as the mean ± standard deviation, and were analyzed by a two-sided Student’s t-test. ^*p < 0.05 and ^***p < 0.001.