Figure 6. Galangin up-regulates Smad4 expression by decreasing hsa-miR-4535 level. (A, B) HS68 cells were treated with different concentrations of H2O2 (0, 100, 200 μM) for 24 h. (C, D) HS68 cells were exposed to H2O2 (200 μM) for 1 h and then treated with galangin (30 μM) for 23 h. The expression of hsa-miR-4535 and Smad4 were detected by qRT-PCR. Values are shown as mean ± SE. Quantification of the results is shown (n = 3) *P < 0.05, **P < 0.01, ***P < 0.001 vs untreated control cells; ##P < 0.01, ###P < 0.001 vs H2O2-treated cells.