Research Paper Volume 13, Issue 23 pp 25342—25364

Figure 6. Galangin up-regulates Smad4 expression by decreasing hsa-miR-4535 level. (A, B) HS68 cells were treated with different concentrations of H₂O₂ (0, 100, 200 μM) for 24 h. (C, D) HS68 cells were exposed to H₂O₂ (200 μM) for 1 h and then treated with galangin (30 μM) for 23 h. The expression of hsa-miR-4535 and Smad4 were detected by qRT-PCR. Values are shown as mean ± SE. Quantification of the results is shown (n = 3) ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 vs untreated control cells; ^##P < 0.01, ^###P < 0.001 vs H₂O₂-treated cells.