Figure 3.MiR-212 inhibited EMT and inactivated the Wnt/β-catenin pathway in NPC cells. (A) The protein levels of Vimentin, N-cadherin and E-cadherin were regulated by miR-212 mimics or inhibitor in C666-1 cells. NC means C666-1 cells with empty sequence. n=3-4. Data were quantified and normalized to the intensity of β-actin (left). (B) The protein levels of Wnt/β-catenin and downstream Cyclin D1 were analyzed by western blot. Both total β-catenin and nuclear localized β-catenin were shown. Lamin-B1 was used as the loading control for nuclear proteins. The western blots (right) were the representative images of 3-4 experiments. Data were quantified and normalized to the intensity of β-actin (left). *p <0.05, **p <0.01.
