Research Paper Volume 14, Issue 8 pp 3400—3415

Figure 2. Expression of Circ_0000423 in cartilage and chondrocyte. (A) Expression of Circ_0000423 in chondrocyte with or without IL-1β induced at different time point; (B) Expression of Circ_0000423 in chondrocyte with or without IL-1β induced at different concentrations; (C) Sanger sequencing was used to illustrate the presence of Circ_0000423; (D) The RT-PCR was used to confirm the presence of Circ_0000423. Divergent primers amplified Circ_0000423 from cDNA, but not from genomic DNA; (E) The expression of Circ_0000423 and Line_0000423 mRNA treated with or without RNase R was measured by RT-qPCR; Data are presented as mean ± SEM. ^*P < 0.05 compared with the control group.