Research Paper Volume 14, Issue 8 pp 3529—3539
NRIP1 aggravates lung injury caused by Pseudomonas aeruginosa in mice by increasing PIAS1 ubiquitination
Figure 6. UBE2I affects TC-1 cells by negatively regulating PIAS1. The PA011-treated TC-1 cells were transfected with si-UBE2I alone or together with si-PIAS1. (A) The protein expression of PIAS1 was analyzed by Western botting. (B) MTT assay was used to analyzed the viability of cells. (C) Apoptosis of cells were detected by flow cytometry. (D, E) Relative content of TNF-α and IL-1β were analyzed by ELISA kits. GAPDH was used as an internal reference. N = 6, *P < 0.01.