Research Paper Volume 14, Issue 8 pp 3652—3665

microRNA-569 inhibits tumor metastasis in pancreatic cancer by directly targeting NUSAP1

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Figure 7. NUSAP1 promoted the migration and invasion of PC cells. (A) Western blot and (B) qRT-PCR were performed to verify transfection efficiency (*p < 0.05). (C) Wound healing assay showed migratory abilities of PC cells after knocking down NUSAP1; (D) Transwell assay indicated migratory abilities of PC cells after knocking down NUSAP1; (E) Transwell assay showed invasive abilities of PC cells after knocking down NUSAP1. GAPDH was used as a loading control in Western blot assay. (* p < 0.05, ** p < 0.01, *** p < 0.001, n = 3, Student’s t-test, means ± 95% CI).