Research Paper Volume 14, Issue 8 pp 3666—3686

Figure 6. Rab10 was a direct target of miR-378a-3p. BUMPT cells were transfected with miR-378a-3p analog (100 nM) and then treated with I(2 hours)/R(2 hours) injury. (A) Putative miR-378a-5p complementary binding sites in the 3’UTR of Rab10 mRNA. (B) RT-qPCR analysis of the expression of Rab10. (C) Immunoblot analysis of Rab10 and β-tubulin. (D) Densitometric analysis of proteins signals. (E) Detection of luciferase activities after miR-378a-3p co-transfection with the 3’UTR luciferase reporter vector of Rab10-MUT or WT. Data are expressed as mean ± SD (n = 6). ^*p < 0.05, I/R with scramble group versus scramble group; ^#p < 0.05, I/R with miR-378a-3p mimics group versus I/R with scramble group or Rab10 WT/miR-378a-3p mimic versus other groups.