Figure 9. Effects of pLXSN and WT-TP53 on the colony formation in soft agar in the presence of 5-Fluorouracil. The effects of pLXSN and WT-TP53 on the colony formation in soft agar were examined. Red squares = MIA-PaCa-2 + pLXSN cells, blue circles = MIA-PaCa-2 + WT-TP53 cells. IC50 is indicated with a purple dotted line and IC25 is indicated with a green dotted line. IC25 is a term to indicate inhibition of colony formation at 25%. These experiments were repeated performed and similar results were observed. The colonies for each cell line were normalized to untreated cells so that the results from the MIA-PaCa-2 + pLXSN and MIA-PaCa-2 + WT-TP53 could be compared. **P < 0.005.