Research Paper Volume 14, Issue 13 pp 5390—5405

Figure 5. Silencing TMEFF1 reversed the promotion effect of MIR503HG knockdown on malignant behaviors of ovarian cancer cells. MIR503HG siRNA was transfected into SKOV3 and OVCAR3 cells alone or together with TMEFF1 siRNA, after transfection for 48 h, (A) the expression of MIR503HG was measured. (B, C) The mRNA and protein levels of TMEFF1 were detected with RT-qPCR and Western blotting. (D, E) CCK-8 and Transwell assays were performed to measure cell proliferation and invasion. (F) Flow cytometry was carried out to evaluate cell apoptosis. (G) The expression of E-cadherin, N-cadherin and Vimentin was analyzed. ^*P < 0.05. n = 6 in each group. Each test was repeated at least three times independently.