Research Paper Volume 14, Issue 13 pp 5449—5463
Long non-coding RNA H19 contributes to spinal cord ischemia/reperfusion injury through increasing neuronal pyroptosis by miR-181a-5p/HMGB1 axis
Figure 6. LncRNA H19 knockdown inhibited OGD/R-induced pyroptosis by downregulating HMGB1. PC12 cells were transfected with sh-H19 alone or together with pcDNA-HMGB1 and then subjected to OGD/R. (A) Relative levels of HMGB1 in transfected PC12 cells following OGD/R exposure. (B–D) The levels of IL-1β, IL-18, TNF-α were determined in transfected PC12 cells following H/R exposure by ELISA. (E–H) The protein levels of NLRP3, ASC, and caspase-1 were determined in transfected PC12 cells by Western blotting. (I) Cell apoptosis rate was identified by TUNEL assay. The results were presented as the mean ± SD. N = 3; *P<0.05.