Research Paper Volume 14, Issue 14 pp 5710—5726

Sofosbuvir induces gene expression for promoting cell proliferation and migration of hepatocellular carcinoma cells


Figure 4. Effects of siRNA against candidate genes on cell proliferation assay in SOF treated OR-6 and Huh 7.5.1 cells. OR-6 (A, B) and Huh 7.5.1 (C, D) cells were transfected with non-targeting siRNA (si-Ctrl) or siRNA against PHOSPHO2-KLHL23, TSNAX-DISC1, TRIM39 and RPP21for 48 hrs. The cells were then treated with SOF 1 μM for 24 hrs. The ATP level in cells were accessed to measure cell proliferation (A, C). In addition, the cells were seeded on 3D culture plates to form tumor sphere in the presence or absence of 1 μM SOF for 24 hrs. The experiments were performed from three independent experiments. (*p < 0.05; **p < 0.01; ***p < 0.001).