Research Paper Volume 14, Issue 15 pp 6028—6046
Ascorbic acid induces salivary gland function through TET2/acetylcholine receptor signaling in aging SAMP1/Klotho (-/-) mice
Figure 4. Effects of ascorbic acid on primary salivary gland cells (PSGCs) isolated from SAMP1/kl +/+ and SAMP1/kl-/- mice. (A–D) PSGS kl+/+ cells isolated from SAMP1/kl +/+ mice were treated with ascorbic acid at the indicated concentrations for 24 h. mRNA and protein expression of salivary gland functional proteins in PSGC kl+/+ cells were determined by qRT–PCR and Western blotting. *p < 0.05. (E–G). Under the same conditions, the mRNA and protein expression of several salivary gland functional proteins under ascorbic acid treatment in PSGC kl-/- cells isolated from SAMP1/kl-/- mice. mRNA and protein expression were determined by qRT–PCR and Western blotting, respectively. *p < 0.05; **p < 0.01 versus the control.