Research Paper Volume 14, Issue 15 pp 6255—6268

Figure 2. cMAP4K2 regulates endothelial angiogenic effects in vitro. (A) HRVECs were transfected with scramble (Scr) siRNA, cMAP4K2 siRNA, null vector, cMAP4K2 overexpressed vector, or left untreated (Ctrl) for 24 h. qRT-PCRs were performed to detect the expression levels of cMAP4K2 (n = 4, ^*P < 0.05 vs. Ctrl group). (B and C) MTT and CCK-8 assays were performed to detect cell viability (n = 4, ^*P < 0.05 vs. Ctrl group). (D and E) Ki67 staining assays were performed to detect cell proliferation (n = 4, ^*P < 0.05 vs. Ctrl group). A representative image and quantification result were shown. Scale bar: 20 μm. (F and G) Transwell assay was performed to detect the migratory ability of HRVECs (n = 4, ^*P < 0.05 vs. Ctrl group). A representative image and quantification result were shown. Scale bar: 50 μm. (H and I) The tube-like structures were observed at 6 h after seeding HRVECs on the Matrigel matrix. The cumulative tube lengths for each field were statistically analyzed (n = 4, ^*P < 0.05 vs. Ctrl group). Scale bar: 100 μm. The significant difference was analyzed by one-way ANOVA followed by the Bonferroni post hoc test.