Research Paper Volume 14, Issue 21 pp 8633—8644
Epigallocatechin-3-gallate protects porcine oocytes against post-ovulatory aging through inhibition of oxidative stress
Figure 2. Epigallocatechin-3-gallate (EGCG) rescues aging-induced oxidative stress. (A) Representative images of glutathione (GSH) in the Fresh, 24 h control, 24 h EGCG, 48 h control, and 48 h EGCG oocytes. (B) Quantified intracellular levels of GSH by relative fluorescence intensity (RFI). *p < 0.05 indicates significant difference. (C) Representative images of reactive oxygen species (ROS) in the Fresh, 24 h control, 24 h EGCG, 48 h control, and 48 h EGCG oocytes. (D) Quantified intracellular levels of ROS by RFI. Scale bars represent 100 μm. Values are presented as the mean ± standard error of the mean (SEM). ****p < 0.001 indicates significant difference. Values are expressed as the mean ± SEM.