Research Paper Volume 14, Issue 21 pp 8805—8817

Figure 2. DSC2 inhibits the cell proliferation ability in vitro and affects cell cycle-related proteins. (A) The clone formation ability of overExp-DSC2-transfected LM3 and shRNA-DSC2-1-transfected 7721 cells was shown by clone formation assay (repeated three times). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001. (B, C) The cell viability of overExp-DSC2-transfected LM3 and shRNA-DSC2-1-transfected 7721 cells were detected by CCK-8 and EdU assay (repeated three times for both). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001. (D) Western blot analysis of the protein levels of Cyclin D1, Cyclin B1, CDK1, and CDK2 from LM3 and 7721 cells transfected with overExp-DSC2 and shRNA-DSC2-1 (repeated three times). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001.