Research Paper Volume 14, Issue 22 pp 9149—9166
LINC00629, a KLF10-responsive lncRNA, promotes the anticancer effects of apigenin by decreasing Mcl1 stability in oral squamous cell carcinoma
Figure 7. The KLF10-LINC00629-Mcl1 axis plays an important role in the anticancer activity of apigenin. (A, B) UM-SCC6 and Cal-27 cells with or without KLF10 knockdown were treated with apigenin as indicated. Cell viability was measured by an MTT assay. (C–E) Apoptosis was analyzed by flow cytometry. (F) LINC00629 was overexpressed in Cal-27 and UM-SCC6 cells with or without KLF10 knockdown, and the cells were treated with 40 μM apigenin for 36 h. The expression levels of Mcl1 and KLF10 were determined by Western blotting. (G, H) Apoptosis was analyzed by flow cytometry. In (A–D, G) the results represent three independent experiments; *p<0.05, **p<0.01, ***p<0.001.