Research Paper Volume 15, Issue 8 pp 2999—3020

Upregulation of RasGRF1 ameliorates spatial cognitive dysfunction in mice after chronic cerebral hypoperfusion

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Figure 1. Experimental procedures and timeline. The rats were treated by 2VO and sham surgery. 21 days (3 weeks) later, the cerebral stereotaxic injection was done. 7 days (1 week) later, the Morris water maze and Y maze were done. 1 day later, electrophysiological experiments were done on rats. Next day, the rats were executed and then be tested by Western blot, RT-PCR, immunohistochemistry, Golgi staining and transmission electron microscopy. Abbreviations: CCH: chronic cerebral hypoperfusion; CSI: cerebral stereotaxic injection; EP: electrophysiology.