Research Paper Volume 15, Issue 11 pp 4699—4713
Pan-cancer analysis identifies LPCATs family as a prognostic biomarker and validation of LPCAT4/WNT/β-catenin/c-JUN/ACSL3 in hepatocellular carcinoma
Figure 7. LPCAT4 increased the expression levels of cholesterol biosynthesis via up-regulating ACSL3. (A) Cholesterol synthesis ability was analyzed in LPCAT4 down-regulation and control group. (B, C) ACSL3 mRNA and protein expression levels were analyzed by RT-PCR and western blot assays, respectively. (D) Overexpression of LPCAT4 increased cholesterol biosynthesis, while knockdown of ACSL3 dismissed this effect. (E) Down-regulation of LPCAT4 decreased cholesterol biosynthesis, while overexpression of ACSL3 could counteract this effect.