Research Paper Volume 15, Issue 11 pp 4699—4713

Figure 8. LPCAT4 regulated ACSL3 expression via WNT/β-catenin/c-JUN signaling pathway. (A) GSEA analysis indicated that WNT signaling pathway was found to be significantly enriched in the high LPCAT4 expression group. (B) Western blot assay was used to examine protein expression level. (C) The three transcription factor-binding sites of c-JUN on potential ACSL3 promoter region were indicated. (D) RT-PCR was used to examine ACSL3 mRNA expression. (E) The ChIP assay was used to validate binding domains of c-JUN in the potential ACSL3 promoter. (F) The luciferase assay was used to confirm which binding sites were functional. (G, H) Western blot assay was used to examine protein expression level.