Research Paper Volume 15, Issue 11 pp 4861—4874
Isoorientin ameliorates H2O2-induced apoptosis and oxidative stress in chondrocytes by regulating MAPK and PI3K/Akt pathways
Figure 1. The effect of ISO or H2O2 on chondrocytes. (A) Identification of primary chondrocytes in SD rats. (A-a) shows the morphology of chondrocytes under light microscopy. (A-b) cellular immunofluorescence detection of ACAN expression. (A-c) cellular immunofluorescence detection of Col2α1 expression. (B) The chemical structure of isoorientin (ISO). (C) Toxicity test of ISO on cell viabilities of chondrocytes. (D) Effect of different concentrations of H2O2 on the viability of chondrocytes. (E) Effects of ISO on H2O2-induced injury chondrocytes viability. Results shown are expressed as means ± SD (n = 6). #p < 0.05 compared with control group, *p < 0.05 compared with OA group.