Research Paper Volume 15, Issue 17 pp 8576—8593

Development of a DNA damage-induced senescence model in osteoarthritic chondrocytes

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Figure 3. Expression of cyclin-dependent kinase inhibitors in Etoposide, and IL-1β treated HACs. HACs were treated with etoposide (blue) at 20 μM for 24 h or IL-1β (red) at 1 ng/mL for the length of the experiment and the expression of cyclin-dependent kinase inhibitors evaluated by RT-qPCR (A, B) and WB (C, D) at day 1 (A, C) and day 8 (B, D). Data are shown as mean ± SD, (n ≥ 3). P-values were calculated by Mann-Whitney test, *p ≤ 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.