Research Paper Volume 15, Issue 17 pp 8812—8832
SUMO1-regulated DBC1 promotes p53-dependent stress-induced apoptosis of lens epithelial cells
Figure 4. DBC1 is modified by SUMO1 at K591 in human lens epithelial cells. (A) Three putative lysine residues for SUMOylation of DBC1 were shown in red bold. (B) FHL124 cells were transfected with HA-tagged wildtype DBC1 (DBC1-WT) or its K-to-R mutants (K591R, K599R, K839R) as indicated. 24 hours after transfection, cell lysates were immunoprecipitated (IP) with anti-SUMO1 antibody and immunoblottings were performed with anti-HA. β-tubulin was used as a reference for loading.