Research Paper Volume 15, Issue 18 pp 9544—9560

Figure 1. Culture and identification of hUC-MSCs. (A) On day 12, hUC-MSCs migrated from the Wharton’s jelly tissue. Scale bar=200 μm. (B) Images of long-scaled hUC-MSCs cultured for the fifth passage. Scale bar=200 μm. (C) Immunofluorescence staining of hUC-MSCs with surface markers CD44, CD73, CD90, and CD105 (green fluorescence). Hoechst 33342 (blue) was utilized to stain the nucleus of hUC-MSCs. Scale bar=50 μm. (D) Toluidine blue staining was used to detect the ability of hUC-MSCs to differentiate into chondrocytes. Scale bar=200 μm. (E) Oil red O staining was used to detect the ability of hUC-MSCs to differentiate into adipocytes. Scale bar=200 μm. (F) Alizarin red S staining was utilised to test the capacity of hUC-MSCs to differentiate into osteoblasts. Scale bar=200 μm.