Research Paper Volume 15, Issue 18 pp 9590—9613
YY1 is regulated by ALKBH5-mediated m6A modification and promotes autophagy and cancer progression through targeting ATG4B
Figure 6. YY1 drives cancer progression and autophagy in an m6A-dependent manner. (A, B) Representative images (left) and the quantification (right) of colony formation (A) and transwell (B) assays showing the growth and migration of AGS cells transfected with Ca-NC, Ca-YY1 or co-transfected with Ca-ALKBH5, Ci-YTHDF1 or Ci-ATG4B. (C, D) Representative images (C) and quantification (D) revealing the immunofluorescence staining intensity with mRFP-GFP-LC3 in AGS cells stably transfected with Ca-NC, Ca-YY1 or co-transfected with Ca-ALKBH5, Ci-YTHDF1 or Ci-ATG4B. Red (RFP+GFP-) puncta represent autolysosomes, and yellow (RFP+GFP+) puncta represent autophagosomes. Scale bar: 10 μm. (E) Representative images from TEM scanning (left panel) and quantification (right panel) exhibiting autophagic vacuoles in AGS cells stably transfected with Ca-NC, Ca-YY1 or co-transfected with Ca-ALKBH5, Ci-YTHDF1 or Ci-ATG4B. Scale bar: 1 μm. *P < 0.05 vs. Ca-NC+Ctrl.