Research Paper Volume 15, Issue 18 pp 9842—9857

Figure 7. COL22A1 knockdown inhibits glioma cell proliferation and migration. (A) RT-qPCR was used to detect the expression of COL22A1, IGF2BP2, and MPO in NHA, U87, LN229, and LN18 cells. (B) IHC was used to detect the expression of COL22A1 in grade 4 diffuse gliomas and adjacent non-tumor tissues. (C) Western blotting was used to detect the expression of COL22A1 in negative control (NC) and COL22A1 knockdown cells. (D) The CCK-8 assay was used to measure proliferation in NC and COL22A1 knockdown cells. (E) Colony formation assays were used to detect tumorigenic potential of NC and COL22A1 knockdown cells. (F) Wound healing assays were used to assess migration rates in NC and COL22A1 knockdown cells. (G) Transwell assays were used to estimate invasion rates in NC and COL22A1 knockdown cells. *, P<0.05; **, P<0.01.