Research Paper Volume 15, Issue 19 pp 10193—10212

Figure 2. Relationship between CK1ε, β-catenin, and the OD of BMSCs. (A) Relative mRNA expressions of CK1ε and β-catenin after the OD of BMSC. (B) Western blot detection and analysis of relative CK1ε and β-catenin protein levels after two weeks’ induction. (C) qRT-PCR was used to detect the relative mRNA expression of CK1ε after transfection. (D) The relative mRNA expression levels of OD-related genes (OCN, RUNX2, SP7, and COL1A1) after CK1ε transfection. (E) ALP activity on the 14th day of BMSC’s OD with different transfections. The experiments in this figure were repeated three times, and similar results were obtained. The data are presented as the means ± SD of independent experiments. The Student’s t-test was used for (A, B) and one-way ANOVA was used for (C–E). ^*P < 0.05; ^**P < 0.01; ^***P < 0.001; Abbreviation: ns: not statistically significant.