Figure 1.Usenamine A inhibits the proliferation, migration, and invasion of LUAD cells. (A) The viability of human LUAD cell lines (H1299 and A549) and normal lung epithelial cells (BEAS-2B) was measured using the CCK-8 assay. (B) H1299 cell proliferation was detected using the colony formation assay. (C) H1299 cell migration was measured using the wound-healing assay. Scale bar = 50 μm. (D) H1299 cell migration and invasion were determined using the transwell assay. Scale bar = 50 μm. (E) H1299 cell apoptosis was assessed by flow cytometry. *p < 0.05 and **p < 0.01 vs. 0 μg/mL usenamine A; #p < 0.05 and ##p < 0.01 vs. 1 μg/mL usenamine A; $p < 0.05 and $$p < 0.01 vs. 2 μg/mL usenamine A.
