Research Paper Volume 16, Issue 6 pp 5336—5353

Figure 5. miR-31-5p can target AMPK in mouse colon tissues. (A) Dual luciferase report assay of AMPK 3′UTR wild-type or mut-type and miR-31-5p. ^**P < 0.01, ^***P < 0.001 vs. Negative Control. (B) Sequence difference between two cloned plasmids: the difference is located in the cloned AMPK DNA fragment, in which the sequence ATTTTGCCC in pmirGLO-AMPK-WT corresponding to the red box is the targeting fragment of mir-31-5p. (C) Wild-type sequences of AMPK for miR-31-5p target. (D) Relative mRNA of AMPK in mice colonic tissue was measured by qPCR (n ≥ 5). Each bar represents mean ± SD. ns represented P > 0.05, ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 vs. DSS group.