Research Paper Volume 16, Issue 6 pp 5336—5353

Antagomir of miR-31-5p modulates macrophage polarization via the AMPK/SIRT1/NLRP3 signaling pathway to protect against DSS-induced colitis in mice

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Figure 6. mir-31-5p may target AMPK/Sirt1/NLRP3 axis to mediate DSS-induced colonic mucosal inflammation in mice. (A) Immunohistochemical analysis of P-AMPK, Sirt1 and NLRP3 in colon of mice. Yellow brown were positive signals (the ruler was 10 um, and the positive signals were marked by red arrows). (B) The relative mRNA levels of AMPK, Sirt1, NLRP3 and caspase-1 in colon tissues of mice were detected by qPCR. (C) Western blot analysis of P-AMPK/AMPK, NLRP3, Sirt1 and caspase-1. (D) Quantitative protein analysis of P-AMPK/AMPK, Sirt1, NLRP3 and caspase-1. Each bar represents mean ± SD, n ≥ 5 from each group. Ns represented P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001 vs. DSS group.