Research Paper Volume 16, Issue 6 pp 5567—5580

Figure 6. CRIF1 overexpression inhibited oxidative stress and senescence in DOX-induced AC16 cells. (A) AC16 cells were transfected with Ov-NC or Ov-CRIF1, and the overexpression efficacy of CRIF1 was detected by western blot. ***P<0.001 vs Ov-NC. (B) DCFH-DA staining was used to detect ROS levels. (C) The expression levels of MDA, GSH and CAT in cells were detected by biochemical kits. (D) SA-β-gal staining was used to observe the level of cell senescence. (E, F) RT-qPCR and western blot were used to detect the expression of p27, p21, and p16. ***P<0.001 vs Control; ##P<0.01, ###P<0.001 vs DOX+Ov-NC.