Research Paper Volume 16, Issue 6 pp 5000—5026

Figure 4. GNL effects of PI3K/AKT signaling in the hippocampus of D-gal-induced mice. (A) pPI3K and pAKT protein analysis by Western blot. Three independent experiments are shown here. SDS-PAGE resolved the protein from each sample, and Western blots were done. The internal load controllers were AKT and PI3K. Densitometry analysis calculated changes in protein bands as 1.0-fold, as shown below the gel. (B) 4- month-old young control and 16-month-old mice hippocampus tissue were analyzed for pPI3K and pAKT levels. The internal load controllers were AKT and PI3K. Densitometry analysis calculated changes in protein bands as 1.0-fold, as shown on the right side of the gel. Values are expressed as the mean ± SD (n = 6). ^*P < 0.05 vs. control group; ^#P < 0.05 vs. D-gal alone-treated mice. ^@P < 0.05 young vs. old mice.