Research Paper Volume 17, Issue 7 pp 1810—1833
Co-regulation of Nr1d1 and Pparα in age-related changes of lipid metabolism and its modulation by calorie restriction
Figure 10. Effects of NR1D1 overexpression on (A) the PPRE binding activity of PPARα and (B) expression changes of circadian and lipid metabolic genes (n = 6 per group). HepG2 cells were transfected with pcDNA or the NR1D1 plasmid. The luciferase reporter assay measures the binding of PPARα to the PPRE element with varying amounts of NR1D1 expression vectors. PPRE binding of PPARα decreases in a NR1D1 dose-dependent manner. Furthermore, the circadian gene BMAL1, CLOCK, PER1/2, and CRY1/2 and lipid metabolic genes ACOX1, CPT2, and PPARA are decreased with Nr1d1 overexpression. Data are presented as mean ± SEM. ###, ***p < 0.001 between two groups. OE; overexpression.