Research Paper Volume 17, Issue 7 pp 1834—1851
Sesn-1 is required for lifespan extension during caloric deprivation in C. elegans through inhibition of mTORC1 and activation of autophagy
Figure 5. Autophagy intensity in C. elegans is influenced by sesn-1 and npp-18, a component of the GATOR2 complex. Control adIs2122 and adIs2122; sesn-1(RNAi) nematodes expressing GFP::LGG-1, with or without simultaneous npp-18 knockdown by RNAi, were subjected to starvation. The relative levels of GFP::LGG-1 conjugation to autophagosomes were measured by immunoblot densitometry. All bar graphs represent blot intensity normalized to actin.