Research Paper Advance Articles
Blood vessel and osteocyte networks concurrently rearrange during bone maturation and decline during aging in the femur of male mice
Figure 2. Blood vessel (BVs) network characterization. (A) GFP+ (green) blood vessels in growing, mature, middle-aged and aged femurs, scale bar = 200 μm. (B) Categorization of the branched vessels used for quantification. (C) Correlation between the number of GFP+ BVs per bone volume and age (months). (D) Correlation between the percentage of volume of BVs per mm3 of cortical bone and age (months). (E) Lectin+ (cyan) blood vessels in cortical bone in growing, mature, middle-aged and aged femurs. * indicates the blood vessel location. The dashed lines delineate bone. Statistical analyses: Spearman’s correlation.