Research Paper Advance Articles
Blood vessel and osteocyte networks concurrently rearrange during bone maturation and decline during aging in the femur of male mice
Figure 3. Osteocyte network age-related changes. (A) Representative images of the osteocyte network labeled with phalloidin (white) and nuclei (in blue). (B) Number of osteocyte nuclei (Ocy) per mm2 of bone (10-μm-thick section) for each group. (C) Correlation between the number of dendrites per osteocyte and age (months). (D) Modelization (in red) of the dendrites used for quantification of the number of dendrites per osteocyte (scale bar = 10 μm). The data are presented as mean ± s.d. Statistical analyses: KW, Dunn’s multiple comparison test with the mature group as reference, Spearman’s correlation, ***p < 0.001.