Research Paper Advance Articles
Blood vessel and osteocyte networks concurrently rearrange during bone maturation and decline during aging in the femur of male mice
Figure 4. Age-related changes for the gene expression and protein synthesis of Podoplanin and Connexin 43. (A) Podoplanin gene expression. (B) Quantification of Podoplanin-positive osteocytes (Ocy) based on immunofluorescence (IF). (C) Representative images for Podoplanin immunofluorescence for the 4 groups. Podoplanin labeling appears in white (filled arrowhead), the empty arrowhead shows a negative cell, and nuclei appear in blue. (D) Gja1 gene expression and correlation with age (months). (E) Quantification of dots/cell body based on immunofluorescence. (F) Representative images for Connexin 43 immunofluorescence, Connexin 43 labeling appears in white, nuclei appear in blue. The data are presented as mean ± s.d. Statistical analyses: KW, Dunn’s multiple comparison test with the mature group as reference, Spearman’s correlation, *p < 0.05, **p< 0.01.