Research Paper Advance Articles

Figure 2. The inverse relationship between DNMT1 and SPINT2 expression is also observed in the OSIS model of primary HDF. (A–C) OSIS was induced by exposing primary HDFs (DT2) to the indicated concentrations of H₂O₂ for 3 days. (A) SA-β-gal activity was quantified (upper panel), and representative staining images are shown (lower panel). ^**p < 0.01 vs. HDF DT2 control (C) by Student’s t-test. (B) qRT-PCR analysis of DNMT1 and SPINT2 mRNA levels. ^**p < 0.01 vs. C by Student’s t-test. (C) Western blot analysis of DNMT1, SPINT2, and p21 protein expression. (D, E) HDFs (DT2) were treated with 200 μM H₂O₂ for the indicated time points. (D) qRT-PCR analysis of DNMT1 and SPINT2 mRNA levels. ^**p < 0.01 vs. control (C) by Student’s t-test. (E) Western blot analysis of DNMT1, SPINT2, and p21 protein expression.