Research Paper Advance Articles

Figure 1. OT+A5i extends the lifespan of old frail male, but not female mice. (A) Outline of the longitudinal study. Old (24–26 mo) C57BL/6J mice were administered by subcutaneous injections with a mixture of OT and A5i (OT+A5i) or HBSS (control) for 2 weeks, followed by 2 weeks of health tests. These rounds were continued for the duration of the natural lifespan. (B) Photos of OT+A5i and control mice. (C) Survival curves of male and female mice that were administered with OT+A5i or control vehicle (Control). Male OT+A5i vs. Control (p = 0.0125). Control n = 12 and OT+A5i n = 14. Female OT+A5i vs. Control (p = 0.1904). Average survival time of female OT+A5i is 115.3 days. Average survival time of female controls is 144.7 days. Control n = 13 and OT+A5i n = 10.