Research Paper Advance Articles

Figure 4. Acute effects of OT+A5i on de novo systemic proteomes of males and females. (A) Schematics of the experimental approach. (B) Uniform Manifold Approximation and Projection (UMAP) plots of the profiles of de-novo synthesized systemically present proteins of young control, old control, and old OT+A5i mice, males and females. AHA and ANL data are merged. (C) Sex-annotated comparison of the mean SDs of the Levene’s test passed proteins (p < 0.05) with lower noise in young control vehicle mice compared to old control vehicle mice and in old mice administered with OT+A5i vs. vehicle control. In the shown line graphs, SDs of all groups are normalized by the SDs of the old vehicle control and presented on a log scale. (D) cirFunMap visualization of KEGG enriched pathways of de-novo synthesized systemic proteomes after OT+A5i treatment. (E) cirFunMap visualization of KEGG enriched pathways of the de-novo synthesized proteins that are noisier in the old as compared to young mice and become youthfully normalized by OT+A5i in old males and females. n = 4 each young and old MetRS^L274G vehicle control, n = 4 each young and old C57.B6 vehicle control, n = 4 old OT+A5i C57.B6.