Research Paper Advance Articles

Figure 1. Genome-wide sequencing of miRNAs in control and HGPS fibroblasts across cellular ages. (A) Representative SA-β-galactosidase-stained cells (10x magnification; scale bar 100 μm) show control fibroblasts at young passages with < 5% senescence and (B) old passages with 15-20% senescence. (C) A total of 66 significantly deregulated miRNAs were identified across six comparisons (control old vs. control young; HGPS old vs. HGPS young; young HGPS vs. young control; old HGPS vs. old control; old HGPS vs. young control; young HGPS vs. old control). Genome-wide miRNA profiles were generated from control cell strains (GM01651, GM01652, GM03349) and HGPS cell strains (HGADFN003, HGADFN127, HGADFN178) at <5% and 15-20% senescence. (Also see Supplementary Table 1).