Research Paper Advance Articles

Figure 1. Differential gene expression in the hypothalamus of SRC-1-KO mice. (A) Diagram depicting mouse brain in sagittal view (top) and coronal view (bottom) with red dotted lines indicating hypothalamic region collected for RNA-seq experiment. (B) Volcano plot of RNA-seq results (center) with log₂ fold change plotted on the x-axis and -log₁₀ corrected p-value (Padj) plotted on the y-axis. Horizontal dashed line indicates Padj = 0.05. Two vertical lines indicate 1.5-fold either up or down. Each dot represents one gene. Genes plotted in red represent genes that are both 1.5-fold different with a Padj < 0.05. Light grey dashed horizontal line marks Padj = 1e-28 and indicates genes plotted above this line are out of the scale of the panel. Large orange arrows mark the number of genes that are 1.5-fold lower (left) or higher (right) with a Padj < 0.05. (C) Gene ontology for molecular function using genes 1.5-fold down with Padj < 0.05 (top) or 1.5-fold up with Padj < 0.05 (bottom). (D, E) Violin plots for S100a6 (D) and S100a11 (D) normalized to transcripts per million (TPM). ^* and ^**, Padj < 0.05 or 0.01. (F) Gene set enrichment analysis using Kyoto encyclopedia of genes and genomes (KEGG) as output with a rank ordered gene list, using log2 fold change X -log10 Padj as the algorithm to rank genes, as input.