Research Paper Advance Articles

Figure 5. Aging-associated changes in the expression of SRC-1, S100A6 and S100A11 in human. Secondary analysis of published RNA-Seq data revealed the differential gene expression between young (20–40 years of age) and old (60–80 years of age) human. (A–D) The expression of SRC-1 coding gene NCOA1, (E–H) S100A6 coding gene and (I–L) S100A11 coding gene in the hippocampus, hypothalamus, cerebellum and cortex of young vs aged human brains, respectively. ^* and ^**P < 0.05 or 0.01 in Wald test from DESeq2 with Benjamini-Hochberg correction for multiple comparisons. (M, N) Western Blot detection (M) and quantification (N) of SRC-1 protein levels compared to housekeeping protein β-actin in the hippocampus of young (3 months of age) and aged mice (13 months of age). N = 4–5 mice. ^*P < 0.05 in unpaired two-tailed t-tests.