Research Paper Advance Articles

Figure 6. The regulation of S100a6 by transcription factors and SRC-1. (A, B) Relative mRNA levels of S100a6 mRNA measured in the hypothalamus (A) and the hippocampus (B) isolated from control vs. SRC-1-KO mice using Q-PCR. Data are presented as presented as mean ± SEM. N = 5 samples per group. ^*P < 0.05, ^**P < 0.01 in unpaired two-tailed t-tests. (C) Binding sites of transcription factors on the promoter of S100a6 and the effect of indicated transcription factor on S100a6 promoter luciferase activity in Neuro 2A cells. Data are presented as mean ± SEM. N = 3–4 repeated experiments with 3 biological replicates per group in each experiment. ^**P < 0.001 in one-way ANOVA analyses followed by Sidak tests. ^#P < 0.05 in unpaired two-tailed t-tests against Control. (D, E) Effects of SRC-1 and transcription factors KLF (D) and AP2 (E) on S100a6 promoter luciferase activity in SRC-1-KO MEF cells. Data are presented as mean ± SEM. N = 3–6 repeated experiments with 6 biological replicates per group in each experiment. Control group is normalized to 1 to allow comparisons among different batches of experiments. ^*P < 0.05, ^**P < 0.01 and ^***P < 0.001 in two-way ANOVA analyses followed by Sidak tests.