Research Paper Advance Articles
Bleomycin promotes cellular senescence and activation of the cGAS-STING pathway without direct effect on fibrosis in an idiopathic pulmonary fibrosis model
Figure 5. γH2AX expression was observed in the perinuclear region of the iUIP PCLS. (A, B) p53 expression was determined using WB in PCLS from normal (A) and iUIP (B) treated with the vehicle or 1 μΜ bleomycin for 48 h. (C) IF staining for γH2AX in PCLS paraffin-embedded sections from normal and iUIP treated with the vehicle or 1 μM bleomycin for 48 h. (D, E) Percentage of γH2AX-positive cells (D) and nuclear γH2AX-positive cells (E) were calculated based on the total number of cells. (F) IHC staining for(γH2AX (red), H2AX (blue), and lamin B1 (green) in PCLS from normal and iUIP treated with the vehicle or 1 μM bleomycin for 48 h. (G, H) Lamin B1 expression was determined using WB in PCLS from normal (G) and iUIP (H) treated with the vehicle or 1 μM bleomycin for 48 h. β-Actin was used as an internal control for WB. The results are shown as mean ± SE of five mouse PCLSs in each group. Asterisks indicate *P < 0.05 and **P < 0.01 compared with the vehicle. “ns”, not statistically significant. Scale bars indicate 50 μm (white) and 10 μm (yellow). Immunohistochemical staining of gH2AX was performed three times independently.