Bleomycin promotes cellular senescence and activation of the cGAS-STING pathway without direct effect on fibrosis in an idiopathic pulmonary fibrosis model

Yoko Miura; Nadia Milad; Akiho Takata; Satoshi Kanazawa

doi:doi:10.18632/aging.206312

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Research Paper Advance Articles

Bleomycin promotes cellular senescence and activation of the cGAS-STING pathway without direct effect on fibrosis in an idiopathic pulmonary fibrosis model

Figure 7. Excessive senescence occurred in iUIP PCLS treated with bleomycin. (A, B) PCLS samples were treated with the vehicle or 1 μM bleomycin for 120 h. P16^INK4A (A) and P19^ARF (B) expression were determined using qPCR. Hprt was used as an internal control for qPCR. The results are shown as mean ± SE of five mouse PCLSs at each stage. Asterisks indicate ^*P < 0.05 and ^**P < 0.01 compared with the vehicle. “ns”, not statistically significant. (C) β-galactosidase staining was detected in PCLS from normal and iUIP was treated with the vehicle or 1 μΜ bleomycin for 48 h. (D) JC-1 staining was detected in PCLS from normal and iUIP was treated with the vehicle or 1 μΜ bleomycin for 4 h. (E) TAFs (TelC, red) were co-localized with γH2AX (green) using IF and telomere in situ hybridization. Scale bars indicate 50 μm (white) and 10 μm (yellow).