Research Paper Advance Articles
Lactobacillus amylovorus KU4 inhibits adipocyte senescence in aged mice through necdin regulation of p53 activity
Figure 2. LKU4 attenuates the susceptibility of adipocytes to age-related cellular senescence. (A) RT-qPCR analysis of cellular senescence-associated gene expression in stromal vascular fraction (SVF) cells and adipocyte fractions from iWAT of 2-month-old and 24-month-old mice (n = 3). (B) RT-qPCR analysis of cellular senescence-associated gene expression in 3T3-L1 preadipocytes (pre) and day 8 3T3-L1 mature adipocytes (mature). Cells were treated with 100 μM H2O2 for 24 h in the absence or presence of LKU4–CM. All data are expressed as the mean ± S.E.M. * p < 0.05, *** p < 0.01.