Research Paper Advance Articles
Runx1 overexpression induces early onset of intervertebral disc degeneration
Figure 5. Runx1 overexpression reduced the accumulation of aggrecan in the NP. (A, C) Representative images of immunohistochemistry staining of aggrecan (A) and ADAMTS5 (C) at 5M, 7M, and 10M. Scale bar = 200 μm. Magnified NP areas are shown. Hematoxylin was used as nuclei staining and immunopositivity was labeled brown. (B, D) Quantification results showing that the expression of aggrecan (B) was significantly decreased at 7M and 10M while the expression of ADAMTS (C) was increased in the NP of Runx1 overexpression mice. n = 5 mice /group. Student’s t-test was performed (* p < 0.05, *** p < 0.001).