Research Paper Advance Articles

Figure 6. TRF1 ablation leads to weight loss and less adiposity in high-fat diet males but not in females. (A) Experimental plan: Trf1^+/+ and Trf1^lox/lox; hUBC-CreERT2 subjected to a high-fat diet at 8 weeks and treated intraperitoneally with tamoxifen at 12 weeks until humane endpoint. (B) Representative images of males and females at 17 months (left), weight (center), fat (g) and percentage of adiposity measured by DEXA (right). Note that Trf1^Δ/Δ males weigh less and have lower fat content and adiposity. (C) Length measurement of intestinal villi (microns). (D) HDL and LDL levels (mg/dL) in serum 18-week-old mice of Trf1^+/+ and Trf1^Δ/Δ in both sexes. (E) Measurement of blood urea nitrogen (BUN) in plasma of male mice. (F) Weight of brown adipose tissue (BAT) of males. (G) Subcutaneous fat layer thickness (um) of males. Error bars, s.e.m.; only significant values are shown; *P < 0.05; **P < 0.01; ***P < 0.001 determined by two-tailed Student’s t-test (B–G)